The strikes staged by approximately 2,000 ground staff have caused several flights to and from Berlin's Schönefeld and Tegel airports this week to be canceled.
Ver.di argued that another walkout was necessary because employers hadn't reacted to the previous strike and made a new offer in their wage dispute.
Berlin's two airports are served by carriers including Air Berlin (AB1.DE), Lufthansa (LHAG.DE), easyJet (EZJ.L) and Ryanair (RYA.I), all of which have been hit by the strikes.
Last Friday and Monday are peak travel days.
A4E said 1,500 flights were cancelled during the five-day strike by staff at control centres in parts of France, which ended on Sunday.
Over 600 flights were expected to be cancelled today. The strike is extended by another 24 hours and will continue until Wednesday, March 15.More news: Arkansas Headed To NCAA Tournament As No. 8 Seed
All flights between Sofia and Berlin on Monday have been cancelled.
The different tasks performed by them are checking in passengers, loading and unloading baggage and different cargoes, along with directing an aircraft which is on the tarmac.
Tigel airport is around eight kilometer away from the city of Berlin and handles more than 21 million passengers an year, while Berlin Shonefeld, situated in the southeast of Berlin, handles more almost 12 million passengers per year.
The strike by ground staff, who want an increase in their hourly pay to 12 euros ($12.71) from 11 euros, is due to run for 25 hours from 0400 local time (0300 GMT) on Friday.
In order to tackle the situation the management is taking some firm steps.
The issue is not yet resolved and people are not willing to get back to the work.